Lecture
Biophysical Chemistry
Dr. Peter J. Walla, Institute for Physical and Theoretical Chemistry
Wintersemester 2006 / 07
The script handed out in the lecture is exclusively for non-commercial, educational purposes! It is not allowed to make copies for people other than participating in the lecture!
Contents
Introduction
What is biophysical chemistry?
- An example from drug screening
Basics in biochemistry
- Structure and function of proteins, DNA and other biomolecules
- Basic insights into cell structure and function
Chapter 1
Techniques for the investigation of biomolecules and biochemical processes
- Optical techniques
Principles and the characteristics of natural chromophores
Bioassays and label techniques
- NMR and ESR
- Mass spectroscopy
- X-Ray techniques
Chapter 2
Mechanisms and questions in biophysical chemistry research
- Enzyme function and kinetics
- Receptor ligand interactions
- ATP, NADP and others and their thermodynamics
- How are nerves and muscles working?
- Photosynthesis
Chapter 3
Special Techniques and Questions
- Ultrafast and nonlinear spectroscopy
- Optical single molecule detection
- Optical tweezers
- Labelfree detection methods
- DNA-Chips, Nanotechnology and other cutting edge technologies
- Atomic force microscopy and other scanning techniques
- Patch-Clamp
Chapter 4
Industrial applications
- High through-put screening
- Reader and Robotics
- Fluorescence assisted cell sorters
Literature
General:
R. Winter and F. Noll Methoden der Biophysikalischen Chemie, B. G. Teubner, Stuttgart (1998)
F. Lottspeich, H. Zorbas Bioanalytik, Spektrum Akademischer Verlag, Heidelberg, Berlin (1998)
K. E. van Holde, W. C. Johnson, and P. S. Ho Principles of Physical Chemistry, Prentice Hall, Upper Saddle River, (1998)
C. R. Cantor and P. R. Schimmel Biophysical Chemistry, W. H. Freeman, San Francisco (1980)
Biophysics text book online, http://www.biophysics.org/education/topics.htm#techniques
- Fluorescence Correlation Spectroscopy. Petra Schwille and Elke Haustein
- Optical Tweezers: Measuring Piconewton Forces. Mark Williams
Biochemistry
J. M. Berg, J. L. Tymoczko, L. Stryer Biochemistry, W. H. Freeman and Company, New York, (2001)
Optical Methods
J. R. Lakowicz Principles of Fluorescence Spectroscopy, Plenum Press, New York & London
H. Haken, H. C. Wolf Atom und Quantenphysik, Springer Verlag, Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong, Barcelona, Budapest
H. Haken, H. C. Wolf Molekülphysik und Quantenchemie, Springer Verlag, Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong, Barcelona, Budapest