BACK

Vorlesung

       "Biophysikalische Chemie"

Dr. Peter J. Walla, Institut für Physikalische und Theoretische Chemie

WS 11/12

Vorlesung: Do., 11.30 Uhr bis 13.00 Uhr   (HR30.1)      

                 Fr., 11.30 Uhr bis 12.15 Uhr   (HR30.1)     

Übung:       Fr., 12.15 Uhr bis 13.00 Uhr  (HR30.1)      

Erste Stunde:    Donnerstag, 27. Oktober 2011

Klausur:  für Chemiker am Dienstag, 14.02., 10.00-13.00 Uhr in HR 30.1

              für Biotechnologen am Dienstag, 06.03., 13.30 Uhr in ZI 24.1

Klausur für Chemiker (14.02.2012):             Ergebnisse

Klausur für Biotechnologen (06.03.2012):    Ergebnisse

Hausaufgaben

Biochemie-Skript

Passwortgeschützes Skript! Passwort wird in der Vorlesung bekanntgegeben!

Skript

Contents

Introduction

    What is biophysical chemistry?

            - An example from drug screening

    Basics in biochemistry   

            - Structure and function of proteins, DNA and other biomolecules   

            - Basic insights into cell structure and function

Chapter 1

    Techniques for the investigation of biomolecules and biochemical processes

            - Optical techniques

                Principles and the characteristics of natural chromophores

                Bioassays and label techniques

            - NMR and ESR

            - Mass spectroscopy

            - X-Ray techniques

Chapter 2

    Mechanisms and questions in biophysical chemistry research

            - Enzyme function and kinetics

            - Receptor ligand interactions

            - ATP, NADP and others and their thermodynamics

            - How are nerves and muscles working?

            - Photosynthesis

Chapter 3

    Special Techniques and Questions

            - Ultrafast and nonlinear spectroscopy

            - Optical single molecule detection

            - Optical tweezers

            - Labelfree detection methods

            - DNA-Chips, Nanotechnology and other cutting edge technologies

            - Atomic force microscopy and other scanning techniques

            - Patch-Clamp

Chapter 4

    Industrial applications

            - High through-put screening

            - Reader and Robotics

            - Fluorescence assisted cell sorters

Literature

General:

Walla Modern Biophysical Chemistry, (2009)

R. Winter and F. Noll Methoden der Biophysikalischen Chemie, B. G. Teubner, Stuttgart (1998)

F. Lottspeich, H. Zorbas Bioanalytik, Spektrum Akademischer Verlag, Heidelberg, Berlin (1998)

K. E. van Holde, W. C. Johnson, and P. S. Ho Principles of Physical Chemistry, Prentice Hall, Upper Saddle River, (1998)

C. R. Cantor and P. R. Schimmel Biophysical Chemistry, W. H. Freeman, San Francisco (1980)

Biophysics text book online, http://www.biophysics.org/education/topics.htm#techniques

    - Fluorescence Correlation Spectroscopy. Petra Schwille and Elke Haustein

    - Optical Tweezers: Measuring Piconewton Forces. Mark Williams

Biochemistry

J. M. Berg, J. L. Tymoczko, L. Stryer Biochemistry, W. H. Freeman and Company, New York, (2001)

Optical Methods

J. R. Lakowicz Principles of Fluorescence Spectroscopy, Plenum Press, New York & London

H. Haken, H. C. Wolf Atom und Quantenphysik, Springer Verlag, Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong, Barcelona, Budapest

H. Haken, H. C. Wolf Molek�lphysik und Quantenchemie, Springer Verlag, Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong, Barcelona, Budapest

BACK